human normal cervical epithelial cell line hcerepic  (ATCC)

Journal: Bioengineered

Article Title: Kinetochore-associated protein 1 promotes the invasion and tumorigenicity of cervical cancer cells via matrix metalloproteinase-2 and matrix metalloproteinase-9

doi: 10.1080/21655979.2022.2061144

Figure Lengend Snippet: Expression of KNTC1 in cervical cancer cell lines. (a) qRT-PCR detected the mRNA expression level of KNTC1 in human normal cervical epithelial cells (HcerEpic) and cervical cancer cell lines C-33A and SiHa cell lines. (b) Western blot detected the protein expression level of KNTC1 in human normal cervical epithelial cells (HcerEpic) and cervical cancer cell lines C-33A and SiHa cell lines. KNTC1, kinetochore-associated protein 1.

Article Snippet: Cervical cancer cell lines SiHa and C-33A, and the human normal cervical epithelial cell line HcerEpic, were purchased from Procell (Wuhan, China).

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Journal: Experimental and Therapeutic Medicine

Article Title: Knockdown of LINC01123 inhibits cell viability, migration and invasion via miR-361-3p/TSPAN1 targeting in cervical cancer

doi: 10.3892/etm.2021.10618

Figure Lengend Snippet: Expression of LINC01123 in CC tissue samples and cells. (A) The expression of LINC01123 was evaluated using The Cancer Genome Atlas database. *P<0.05. (B) LINC01123 expression was detected by RT-qPCR in CC and adjacent healthy cervical tissue samples. P<0.001. (C) LINC01123 expression was detected by RT-qPCR in CC tissue samples with or without lymph node metastasis. P<0.01. (D) LINC01123 expression was detected by RT-qPCR in CC cell lines (HeLa, CaSki, SiHa and C-33A) and normal cervical epithelial cell line (HCerEpiC). **P<0.01 vs. HCerEpiC. Experiments were performed in triplicate and repeated at least three times. LINC01123, long intergenic non-protein coding RNA 1123; CC, cervical cancer; RT-qPCR, reverse transcription-quantitative PCR; CESC, cervical squamous cell carcinoma and endocervical adenocarcinoma.

Article Snippet: Cell culture and transfection Human CC cell lines (HeLa, CaSki, SiHa and C-33A) and the human normal cervical epithelial cell line (HCerEpiC) were purchased from American Type Culture Collection.

Techniques: Expressing, Quantitative RT-PCR, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Experimental and Therapeutic Medicine

Article Title: Knockdown of LINC01123 inhibits cell viability, migration and invasion via miR-361-3p/TSPAN1 targeting in cervical cancer

doi: 10.3892/etm.2021.10618

Figure Lengend Snippet: miR-361-3p acted as the direct target of LINC01123. (A) The possible binding site between LINC01123 and miR-361-3p was predicted by LncBase Predicted v.2. (B) The expression of miR-361-3p was detected by RT-qPCR in HeLa and CaSki cells transfected with sh-LINC01123-1 or sh-NC. **P<0.01 vs. sh-NC. (C) Dual-luciferase reporter assay confirmed the association between LINC01123 and miR-361-3p in HeLa and CaSki cells transfected with miR-NC, miR-361-3p mimics, LINC01123-WT or LINC01123-MUT. **P<0.01 vs. miR-NC. (D) miR-361-3p expression was detected by RT-qPCR in CC and adjacent healthy cervical tissue samples. P<0.001. (E) The correlation between LINC01123 and miR-361-3p was evaluated by Pearson's correlation analysis. P<0.0001. (F) The expression of miR-316-3p was detected by RT-qPCR in CC cell lines and HCerEpiC cells. **P<0.01 vs. HCerEpiC cells. The experiments were performed in triplicate and repeated at least three times. miR-361-3p, microRNA-361-3p; LINC01123, long intergenic non-protein coding RNA 1123; CC, cervical cancer; RT-qPCR, reverse transcription-quantitative PCR; sh-NC, short hairpin RNA negative control; WT, wild-type; MUT, mutant.

Article Snippet: Cell culture and transfection Human CC cell lines (HeLa, CaSki, SiHa and C-33A) and the human normal cervical epithelial cell line (HCerEpiC) were purchased from American Type Culture Collection.

Techniques: Binding Assay, Expressing, Quantitative RT-PCR, Transfection, Luciferase, Reporter Assay, Reverse Transcription, Real-time Polymerase Chain Reaction, shRNA, Negative Control, Mutagenesis